regresi=meramalkan nilai var dependen dr var independen dg tk signifikansi/kebenaran ttt
Polyclonal antibodies --> derived from different B cell lines. They are a mixture of immunoglobulin molecules secreted against a specific antigen, each recognizing a different epitope
Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are identical because they are produced by one type of immune cell that are all clones of a single parent cell. Given almost any substance, it is possible to create monoclonal antibodies that specifically bind to that substance; they can then serve to detect or purify that substance.
monospesifik-->sdkt perubahan pd antigen, g terdeteksi o/ antibodi, pdhl kemngknan perubahn krn rad bebas gede
PAbs are heterogeneous and recognize a
host of antigenic epitopes, the effect of change on a single
or small number of epitopes is less likely to be significant.
PAbs are also more stable over a broad pH and salt concentration,
PAbs frequently have better specificity than MAbs because
they are produced by a large number of B cell clones
each generating antibodies to a specific epitope, and polyclonal
sera are a composite of antibodies with unique specificities.
mono activation requires the close proximity
of Fc receptors. Modification of antibodies by covalently
linking a fluorochrome or radionuclide may also alter antibody
binding. This potential is less of a concern when using
PAbs, which recognize a host of epitopes, but it can be
significant for MAbs if the change affects its monospecific
elisa-->The capture and primary antibodies
must be different isotypes, if not from different species, so
that the secondary antibody will only detect the presence of
the primary antibody and correctly indicate that the antigen
has been captured in the well.
igfbp fsx beda2 ta?
Increased IGFBP-2 and decreased IGF-I and IGFBP-3 may be associated with the increased proliferation rate of transplanted bone marrow.
dapus no 1: insulin-like growth factor I (IGF-1)-induced cardiac contractile response in ventricular myocytes from sucrose-fed rats.
Prediabetic insulin resistance is not permissive to the development of cardiac resistance to insulin-like growth factor I in ventricular myocytes
hipoxia-->igfbp naik-->ikat igf1-->pertumb terhambat
kurang gh-->distribusi igf di igfbp berubah-->igf1 & igfbp3 bkrg
dapus no 7: The Phytoestrogen Quercetin Impairs Steroidogenesis and Angiogenesis in Swine Granulosa Cells In Vitro
yg berinteraksi sama igfbp3??
in part through the local IGF-IGFBP system, and
IGFBP secreted by muscle cells may play an important
autocrine/paracrine role in muscle growth and development.
prostatic kallikreins (prostate-specific antigen) hK2 and hK3 may influence specifically the tumoral growth of prostate cells through the degradation of IGFBPs, to increase IGF bioavailability.